Propagation of Canadian rose ‘John Franklin’ in vitro culture

Abstract

Many researchers are interested in optimizing the clonal micropropagation of ornamental plants. It is known that the varietal specificity of the studied crops makes it necessary to select and optimize the composition of the nutrient medium for each stage of propagation in vitro. The purpose of our research was to identify the effect of the composition and concentration of nutrient hormones on multiple shoot formation, root development in regenerating plants of the rose 'John Franklin' in vitro. A technology has been developed for obtaining a large amount of self-rooted planting material of a winter-hardy decorative rose variety in vitro culture. Clonal micropropagation was performed using vegetative buds as explants. The multiple of shoots is achieved by activating the axillary meristems of the shoots. It has been shown that the efficiency of micropropagation of the 'John Franklin' rose increases on the Murashige and Skoog nutrient medium containing BAP at a concentration of 1.0 mg/l and IAA – 0.5 mg/l. This medium provides high quality regeneration of micro-shoots with a propagation coefficient equal to 5.2. The optimal modification of the nutrient medium at the rooting stage was revealed, and the expediency of using IBA with a concentration of 0.5 mg/l as auxin was noted. The selected conditions allow you to get a larger number of roots on the shoot – 5 pcs. and their greatest length is 51.5 mm.