PROPAGATION OF ANTIMICROBIAL RESISTANT Salmonella spp. IN BIVALVE MOLLUSKS FROM ESTUARY AREAS OF BAHIA, BRAZIL

Carla Silva Da Silveira,Oscarina Viana De Sousa,Norma Suely Evangelista-Barreto

doi:10.1590/1983-21252016v29n222rc

Carla Silva Da Silveira, Oscarina Viana De Sousa + Show 1 more

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https://doi.org/10.1590/1983-21252016v29n222rc

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Abstract

ABSTRACT: In recent years, the emergence of resistant pathogens has complicated the treatment of bacterial infections in livestock production as well as in the medical field, due to the development of resistance mechanisms by microorganisms. The objective of this study was to delineate the antimicrobial resistance profile of Salmonella spp. strains isolated from bivalve mollusks (oysters and mussels) and from estuarine environment water of two regions of Bahia, Brazil. Twenty-seven strains, 12 isolated from bivalve mollusks and 15 from estuarine water, were tested. Eight antimicrobial agents (phenicol, beta-lactams, tetracyclines, quinolones and fluoroquinolones classes) were used for a susceptibility test, Minimum Inhibitory Concentration (MIC) and extended-spectrum beta-lactamases (ESBLs) production. Isolates showed high susceptibility to the classes of antimicrobial agents tested, with resistance only to nalidixic acid (27%), ampicillin (25%) and tetracycline (25%). Bacterial resistance was of chromosomal origin and the multidrug resistance index (MAR) among isolates of shellfish (mussels in natura) was 0.25. The MIC was found to be 100 µg/mL, 500 µg/mL and 350 µg/mL to nalidixic acid, ampicillin and tetracycline, respectively. None of the isolates presented ESBLs production. The presence of multidrug-resistant and high MIC Salmonella spp. is being conveyed in extraction areas of bivalve mollusks in the State of Bahia, Brazil.

Highlights

Non-typhoid Salmonella is an important foodborne pathogen that can cause gastroenteritis and sepsis in humans
In bivalve mollusks, whose feeding system is by filtration, high microbial load in tissues puts this food on a risk class for the consumer (AMAGLIANI et al, 2012), as they are very appreciated in natura
Among the pathogenic microorganisms involved in environmental contamination, Salmonella spp. stands out for its wide distribution in nature, being able to be inserted into the aquatic environment through improper disposal of solid waste of human and/or animal origin (BUDIATI et al, 2013)

Summary

Introduction

Non-typhoid Salmonella is an important foodborne pathogen that can cause gastroenteritis and sepsis in humans. Food of animal origin play an important route of transmission of Salmonella and in humans infections are almost always result from the ingestion of food contaminated with bacteria (LAI et al, 2014). Oysters are able to concentrate about three to 62 times more contaminants in their tissues than the environment where they are (MORRISON et al, 2011). Among the pathogenic microorganisms involved in environmental contamination, Salmonella spp. stands out for its wide distribution in nature, being able to be inserted into the aquatic environment through improper disposal of solid waste of human and/or animal origin (BUDIATI et al, 2013). The genus Salmonella comprises more than 2,610 different serotypes (GUIBOURDENCHE et al, 2010). Salmonella enterica serovar Enteritidis has been the leading cause of outbreaks in many African, Asian, European and Latin American countries, being second most common serotype in North America and Oceania, representing 43.5% of all Salmonella isolates (CAMPIONI et al, 2012)

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