Abstract
A 520 base pair DNA segment was excised from the bacteriophage λ-genome by cleavage with the bacterial restriction endonuclease, endo R. HindIII. This segment was covalently joined in vitro to an 880 base pair simian virus 40 (SV40) DNA segment which contains the initiation site for SV40 DNA replication. The latter segment was derived from the genome of a defective reiteration mutant of SV40 also by endo R. HindIII cleavage. When the recombinant molecule, together with wild-type SV40 DNA as helper, was introduced into monkey cells by DNA infection, replication of the λ-DNA sequences was observed, and hybrid genomes were encapsidated into progeny SV40 virions. The structure of the λ-DNA segment after serial passage in monkey cells was examined by use of restriction endonucleases and electron microscopic heteroduplex analysis.
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