Abstract
Abstract As awareness and interest in canebrake restoration activities in the southeastern United States continues to grow, we are faced with numerous challenges. The greatest of these challenges is obtaining enough germplasm to support restoration activities. Three propagation methods are reported in an effort to begin to elicit information regarding propagation of rivercane. Experiments to optimize micro-propagation, seed germination, and macro-propagation methods were compared. Tissue culture (rapid clonal multiplication) has great potential for production of large numbers of propagules. Production of shoots has been optimized, using 4-6 mm diameter explants on MS media, containing 0.1 µM IBA (indole-3-butyric acid ) and 0.01 µM TDZ (thidiazuron). However, the process is hampered by the current inability to generate roots. Maximizing seed germination offers the ability to exploit seed produced on existing canebrakes. Of the six temperature regimes tested on two populations, maximum germination occurred...
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