ProP-PD for proteome-wide motif-mediated interaction discovery

Abstract

Many protein–protein interactions are mediated by short linear motifs (SLiMs; 3–10 amino acid stretches; typically found in intrinsically disordered regions; low-to-mid micromolar affinities). The human proteome is expected to contain tens of thousands of SLiMs. However, to date only ~3000 SLiM instances have been discovered. The field required a scalable and accurate experimental approach to tackle SLiM discovery. Proteomic peptide-phage display (ProP-PD) is based on classical phage display updated with a combination of computational library design, custom oligonucleotide library synthesis, next-generation sequencing (NGS), and integrative data analysis.