Abstract
Activated charcoal (AC, 0.25–1%) added to Murashige and Skoog medium supplemented with coconut milk (CM, 15%), inhibited the androgenic response of Datura metel anthers excised from fresh as well as cold pretreated buds (3 days at 3±2 °C). The inhibitory effect of AC was directly proportional to its concentration for fresh as well as cold pretreated explants. This indicated that either AC itself is toxic or reduces the response by adsorbing some substance(s) from explants and/or from the medium. It was thought that AC is probably reducing the response by adsorbing some active component(s) of CM. This possibility was tested by studying the effect of different concentrations of CM in the presence (1%) and absence of AC. It was found that AC reduced the response only in the presence of CM, which was below the level recorded for anthers incubated on basal medium alone. Individually, both CM and AC enhanced the plantlet yield.
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