Promotion of testosterone propionate on repair of rat peripheral nerve defects with chemically extracted nerves

Abstract

Objective To observe the promotion of testosterone propionate on repair of SD rat peripheral nerve defects with chemically extracted nerves. Methods A total of 60 adult formale SD rats were randomly divided into three groups,20 rats in each group. Group A:l. 0 cm sciatic nerve defect of SD rats were bridged by extracted nerves, and at the same time testosterone propionate was injected into the triceps muscle of calf (50 g/L,0.1 ml,BiW). Group B:The nerve defects of SD rats were repaired by extracted nerves,and sodium chloride was injected (0.1 ml,BiW). Group C;the nerve defects were bridged by auto sciatic nerve. The recovery rate of motor nerve conduction velocities (RRMNCV) and the complex muscular action potential (RRCMAP) in each group were measured at the 16th week after operation. The recovery rate of myelinated fiber populations (RRMFP) was tested. The injured side triceps surae wet weight (ITSWW) and uninjured side triceps surae wet weight (UTSWW) in groups A,B and C were compaired. Results At the 16th week, RRMNCV in groups A, B and C were (59. 06 ± 10. 33)% , (45. 80 ± 9.05)% ,and (23.45 ±4. 60)% correspondingly. There were statistical differences among groups A,B and C (P<0.05).RRCMAP in groups A,B and C was (76. 64 ±5. 01)%, (64. 55 ±4. 20)% ,and (20.44 ±3. 82)% correspondingly. There were statistical differences among groups A,B and C (P<0.05). RRMFP in groups A,B and C were (79. 11 ± 2. 55 )% , (63.67 ±4. 97)%,and (29.76 ± 5.68)% correspondingly. There were statistical differences among groups A, B and C (P<0. 05). ITSWW/UTSWW in groups A,B and C was (70.37 ± 9. 54)% , (56. 77 ±6.08)%,and (33.75 ± 7. 89) % correspondingly. There were statistical differences among groups A, B and C (P<0.05 ). The results showed that group A had the best repairing effects,then group B,and group C the worst. Conclusion Chemically extracted nerves by glycerine can be used to repair SD rat sciatic nerve defects,and have a certain effects. Testosterone propionate can promote this effect. Key words: Peripheral nerve; Nerve defect; Testosterone propionate