Promotion of root emergence in vitro from rhizome buds of Lapageria rosea cv. Nashcourt after proliferation in the presence of paclobutrazol

Abstract

Medium components that may influence the emergence of adventitious root primordia in rhizome buds of Lapageria rosea (Ruiz et Pav.) cv. Nashcourt in vitro were examined. Sucrose was the only medium component tested that affected root emergence. Increasing the sucrose concentration in the medium from 10 to 30 g 1-1 stimulated adventitious root emergence from rhizome buds that had been proliferated on a medium containing the gibberellin biosynthesis inhibitor paclobutrazol (5 μM). Successful clonal propagation of L. rosea cv. Nashcourt can now be achieved by a rhizome bud proliferation stage(s) in the presence of paclobutrazol followed by an adventitious root emergence stage in the absence of paclobutrazol.