Promotion of Keratinocyte Proliferation by Tracheloside through ERK1/2 Stimulation.

Jaegoo Kim,Yu-Kyong Shin,Ki-Young Kim

doi:10.1155/2018/4580627

Jaegoo Kim, Yu-Kyong Shin + Show 1 more

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https://doi.org/10.1155/2018/4580627

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Abstract

Cell migration and proliferation are important for proper wound healing after skin injury. Recent studies have shown that compounds from plants could promote cell migration and proliferation. Tracheloside, which is a plant lignan, has been found to promote the growth of HaCaT cells over 40% compared to other compounds tested based on a cell proliferation assay. An in vitro scratch assay confirmed the healing activity of tracheloside (more than 2-fold increased healing activity after 24 hours of treatment compared with the control) and revealed that this activity is better than that of allantoin (1.2-fold increased after 24 hours of treatment compared with the control), a positive control. With western blot results, wound healing with tracheloside occurred through the phosphorylation of ERK1/2. Therefore, tracheloside is a good candidate to promote wound healing and could be developed as a therapeutic agent for wound treatment or used as a leading compound with higher activity.

Highlights

Skin wound healing is a complex process involving the reepithelialization of missing cellular structures and tissue layers through three phases: inflammation, proliferation, and remodeling
Of the many cell types required during the wound healing process, keratinocytes are important for epithelialization in the proliferative phase as they are the predominant cell of the outermost layer of the skin
We showed the effect of tracheloside on keratinocyte proliferation

Summary

Introduction

Skin wound healing is a complex process involving the reepithelialization of missing cellular structures and tissue layers through three phases: inflammation, proliferation, and remodeling. Of the many cell types required during the wound healing process, keratinocytes are important for epithelialization in the proliferative phase as they are the predominant cell of the outermost layer of the skin. Complex interactions and cross communication between keratinocytes and other cell types during all three phases of wound healing are critical for successful wound closure and repair [1, 2]. Cell proliferation is activated by growth factors and cytokines that are released into the injury site. Combined key events such as signaling, cytoskeletal reorganization, and adhesion processes are required [4]

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