Promotion of DNA renaturation by a non-DNA factor in crown call tumor DNA preparation.

Abstract

A 5400-fold excess of tobacco crown gall tumor DNA increased the renaturation rate of Agrobacterium tumefaciens DNA, whereas, the same excess of healthy plant DNA had no effect on the rate or kinetics of renaturation. Since deoxyribonuclease treatment of the tumor DNA did not remove its ability to accelerate renaturation, the tumor tissue contains a non-DNA factor that increases the rate of renaturation of A. tumefaciens DNA.