Promotion of differentiation of newt primary spermatocytes into spermatids by mammalian FSH via Sertoli cells.

Abstract

Our previous studies showed that mammalian follicle-stimulating hormone (FSH) promoted the differentiation of primary spermatocytes into elongated spermatids in organ culture of testes fragments from Cynops pyrrhogaster. To elucidate the mechanism of FSH action, in this study the testes in the primary spermatocyte stage were dissociated and fractionated into germ and somatic cells, the latter comprising more than 80% Sertoli cells. Radioreceptor assays showed that FSH bound to somatic cells, very probably Sertoli cells, but not to germ cells. FSH elevated intracellular cyclic AMP levels in somatic but not germ cells. In cultures of cell aggregates somatic cells stimulated the differentiation of primary spermatocytes into round spermatids even in the absence of FSH, but to a greater extent in the presence of FSH. However, in the absence of somatic cells the extent of differentiation was similar, irrespective of the presence of FSH. Luteinizing hormone (LH) had no stimulatory effects. Most, if not all, of the somatic cells adherent to germ cells were Sertoli cells based on the criterion that they contained lipid droplets. This indicates that FSH stimulates the differentiation of primary spermatocytes via Sertoli cells. To examine if direct contact between Sertoli cells and germ cells is required for the promotion of differentiation, Sertoli and germ cells were cultured in two different compartments which were separated by a permeable membrane. Under these conditions Sertoli cells did not promote the differentiation of primary spermatocytes either in the absence or presence of FSH, indicating that direct contact between germ and Sertoli cells is required for Sertoli cells to promote the differentiation of primary spermatocytes.