Abstract
In this article, we proposed a facile protocol to functionalize porous calcium phosphate ceramics (PCPC) using dietary tea polyphenols (TP). TP molecules was attracted and anchored by Ca2+ ions from the surface of CPC. These TP molecules modulated the nucleation and crystallization of calcium phosphate nanorods assemblies on the surface of PCPC. Our results prove that these calcium phosphate nanorods assemblies accompanies functional groups of TP make PCPC/TP effectively promote proliferation and differentiation of bone mesenchymal stem cells (BMSCs). We inferred that these calcium phosphate nanorods assemblies might change the surface microenvironment of PCPC, which is critical to promote the proliferation and differentiation of BMSCs. Compared with naked PCPC, PCPC/TP obviously increased BMP2, ErK/MAPK and JNK/MAPK level and mineralization capacity of cells (ALP level).
Highlights
Clinical bone defects are common orthopedic diseases
While the surface of porous calcium phosphate ceramics (PCPC)/tea polyphenols (TP) is distributed with nanorods assembly (Fig. 1d), the magnified image in Fig. 1f further present the detailed structure of those nanorods are about 1.5 lm long and 20 nm in diameter
Our result showed that PCPC/TP further increased the level of bone morphogenetic protein-2 (BMP2) compared with PCPC (Fig. 5)
Summary
Clinical bone defects are common orthopedic diseases. Bone defects are usually treated by transplantation, including autologous bone and artificial bone material. Calcium phosphate ceramics (CPC) are frequently used as bone substitute materials because of their chemical similarity to bone mineral and their desirable characteristics, such as high bioactivity, osteoconduction and even osteoinduction [1,2,3,4,5,6]. It is reported the porous calcium phosphate can strongly adsorb bone-growth related proteins [7, 8], such as bone morphogenetic protein-2 (BMP2). The capacity of osteogenic differentiation is a key issue in the study of bone defect repair [21]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
