Promoting neural cell proliferation and differentiation by incorporating lignin into electrospun poly(vinyl alcohol) and poly(glycerol sebacate) fibers.

Abstract

Electrospinning of natural and synthetic polymers open a new practical approach to tissue engineering by producing fibers. In this study, aligned electrospun poly(vinyl alcohol) (PVA)-poly(glycerol sebacate) (PGS) fibers with various percentages of lignin (0, 1, 3, and 5%wt) fabricated for nerve tissue engineering. The effect of the different amount of lignin on the morphology and diameter of the fibers was investigated by scanning electron microscopy (SEM). The physicochemical properties of fibers were studied using FTIR, tensile strain, contact angle, water uptake, and degradation test. MTT assay and SEM were employed to evaluate PC12 cell proliferation and adhesion, respectively. Immunocytochemistry and gene expression were utilized to study how the lignin affected on cell differentiation. The results revealed the smooth with a uniform diameter of the fabricated fibers, and the increased amount of lignin reduced the fiber diameter from 530 to 370 nm. The modulus of elasticity increased from 0.1 to 0.4 MPa by increasing the lignin percentage. The PC12 cell culture indicated that the lignin enhanced cell proliferation. The mRNA expression level for Gfap, β-Tub III, and Map2 and immunocytochemistry (Map2) revealed the positive effect of lignin on neural cell differentiation. Finally, the results suggest PVA-PGS/5% lignin as a promising material for nerve tissue engineering.