Abstract
Pinostrobin (PI), a natural flavonoid found in a variety of plants, is well known for its rich pharmacological activities. However, its osteogenic function remains unclear. The aim of this study is to evaluate the effect of PI on the proliferation, differentiation, and mineralization of murine pre-osteoblastic MC3T3-E1 cells in vitro using MTT, alkaline phosphatase (ALP) activity, the synthesis of collagen I (Col I) assay, and Von-Kossa staining, respectively. The expression of osteocalcin (OCN) mRNA in cells was detected by real-time PCR. The effect of PI on the differentiation of dexamethasone (DEX)-suppressed cells was also investigated. The results showed that PI greatly promoted the proliferation of MC3T3-E1 cells at 5–80 μg/mL (p < 0.05 or p < 0.01), and caused a significant elevation of ALP activity, Col I content, and mineralization of osteoblasts at 10–40 μg/mL (p < 0.05 or p < 0.01), and the expression levels of OCN gene were greatly upregulated after PI treatment (p < 0.01). Furthermore, PI could rescue the inhibition effect of cell differentiation induced by DEX. Taken together, these results indicated that PI could directly promote proliferation, differentiation, and mineralization of MC3T3-E1 cells and has potential for use as a natural treatment for osteoporosis.
Highlights
Osteoporosis (OP) is a bone disease that is common in the aging population and among post-menopausal women, characterized by low bone mass and micro-architectural deterioration of bone tissue, rendering the individual highly susceptible to fragility fractures [1,2]
The purpose of this work was to investigate the effects of PI on the proliferation, differentiation, The purpose ofofthis work wascell to investigate theFurthermore, effects of PI on proliferation, differentiation, and mineralization osteoblastic lines in vitro
Isoflavonoids like genistein and daidzein extracted from plants have been confirmed to Isoflavonoids like genistein and daidzein extracted from plants have been confirmed to fight fight osteoporosis and promote
Summary
Osteoporosis (OP) is a bone disease that is common in the aging population and among post-menopausal women, characterized by low bone mass and micro-architectural deterioration of bone tissue, rendering the individual highly susceptible to fragility fractures [1,2]. Causes of osteoporosis include estrogen deficiency, genetic disorder, nutritional deficiencies, chronic diseases, chronic exposure to medications, and aging [3]. With the extensive use of glucocorticoids, glucocorticoid-induced osteoporosis has become one of the most distinct and serious adverse effects associated with glucocorticoid therapy. Available therapies for osteoporosis include estrogens, selective estrogen receptor modulators, bisphosphonates, and calcitonin [4]. Hormone (estrogen) replacement therapy (HRT) has been shown to be effective in both preventing postmenopausal osteoporosis and decreasing fracture incidence [5]. It has been recently reported that some naturally occurring compounds induce MC3T3-E1 cell differentiation and have potential activity against osteoporosis [8,9,10,11]. It has been reported that naringin, a plant-derived compound with a flavonone skeleton [12,13], has bioactivity against osteoporosis [14,15,16]
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