Abstract
Traditionally, muscle cell lines are cultured on glass coverslips and differentiated to investigate myoblast fusion and differentiation. Efficient differentiation of myoblasts produces a dense network of myotubes with the correct organisation for contraction. Here we have tested the ability of artificially generated, precisely controlled peptide surfaces to enhance the efficiency of myoblast differentiation. We focused on specific short peptides from α-laminin-2 (IKVSV, VQLRNGFPYFSY and GLLFYMARINHA) as well as residues 15–155 from FGF1. We tested if these peptides in isolation, and/or in combination promoted muscle differentiation in culture, by promoting fusion and/or by improving sarcomere organisation. The majority of these peptides promoted fusion and differentiation in two different mouse myogenic cell lines and in primary human myoblasts. The additive effects of all four peptides gave the best results for both mouse cell lines tested, while primary human cell cultures differentiated equally well on most peptide surfaces tested. These data show that a mixture of short biomimetic peptides can reliably promote differentiation in mouse and human myoblasts.
Highlights
Muscle cell lines, originally derived from satellite cells are commonly used to study muscle fusion and differentiation in tissue culture
We have previously shown that myoblast survival and fusion into myotubes depends on laminin 111, and not on fibronectin or vitronectin (Clark et al 1997)
A single peptide or a mixture of different peptides can be used. This approach allows precise control over concentration and make-up of ECM peptide motifs presented to the cells. We have used this approach to test the abilities of the three LAMA2 peptides described above (Fig. 1; Table 1), together with an FGF1 peptide in promoting myoblast fusion and differentiation
Summary
Originally derived from satellite cells are commonly used to study muscle fusion and differentiation in tissue culture. We have used this approach to test the abilities of the three LAMA2 peptides described above (Fig. 1; Table 1), together with an FGF1 peptide in promoting myoblast fusion and differentiation.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
