Promoting Breast Cancer Cell Invasion by Matrix Metalloproteinase-26 in Novel Threedimensional PVA Sponge Culture System

Abstract

Abstract : Cells are grown in 3-dimensional environments under physiological and pathological condition. While most of the experiments were done using the monolayer cell culture. Accumulating results showed that the expression patterns of genes and proteins are distinctly different in three-dimensional (3D) cultures when compared with the monolayer (2D) cultures commonly employed. Therefore, we created a novel Polyvinyl alcohol (PVA)3D cell culture system to study human breast cancer cell progression and cancer cell-stromal cell interaction. The results showed that both breast cancer cell (MDA-MB-23l)and human fibroblast cell (HFL-l)attachment varied with the different extra cellular matrix components. When the MDA-MB-23l cell invaded into the bottom layer, which contained HFL-l cell in the PVA 3D cell culture, the cells formed breast cancer tissue-like structure. Furthermore, the MDA-MB-23l cell could be easily identified immunohistochemistry using proper epithelial marker. Therefore, This 3D culture system may be a useful medol to study cancer cell-stromal cell interaction and cancer cells invasion. The role of the novel MMP-26 in breast cancer invasion using this 3D cell culture system is under investigation.