Abstract
The DNA sequences, transcription patterns and genetic behaviours of the wild-type his3 gene from Saccharomyces cerevisiae (yeast) and of seven mutations that delete sequences flanking the 5′-end of the messenger RNA coding region are described. The deleted derivatives behave as promoter mutants because they are cis-dominant and fail to make detectable levels of his3 mRNA. The results indicate that sequences further than 44 base-pairs upstream from the mRNA coding region are necessary for proper his3 expression. The seven derivatives that inactivate his3 promoter function in yeast cells also delete the promoter sequence necessary for expression in Escherichia coli.
Published Version
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