Abstract
The carotenoid-derived volatile β-ionone makes an important contribution to tea fragrance. Here, we qualitatively and quantitatively analysed 15 carotenoids in tea leaves of 13 cultivars by UHPLC-APCI-MS/MS. The 13 cultivars were divided into two groups by PCA (Principal Component Analysis) clustering analysis of their carotenoid content, and OPLS-DA (Orthogonal projections to latent structures) indicated that the levels of β-carotene (VIP = 2.89) and lutein (VIP = 2.30) were responsible for much of the variation between the two groups. Interestingly, the β-carotene to β-ionone conversion rates in Group 1 were higher than in Group 2, while the β-carotene content was significantly lower in Group 1 than in Group 2. The β-ionone content was significantly higher in Group 1. Pearson Correlation Coefficient calculation between the transcription level of candidate genes (CsCCD1 and CsCCD4) and the accumulation of β-ionone indicated that CsCCD1 may involve in the formation of β-ionone in 13 cultivars. Prokaryotic expression and in vitro enzyme activity assays showed that ‘Chuanhuang 1’ had an amino acid mutation in carotenoid cleavage dioxygenases 1 (CsCCD1) compared with ‘Shuchazao’, resulting in a significantly higher β-ionone content in ‘Chuanhuang 1’. Sequence analysis showed that ‘Chuanhuang 1’ and ‘Huangdan’ had different CsCCD1 promoter sequences, leading to significantly higher CsCCD1 expression and β-ionone accumulation in ‘Chuanhuang 1’. These results indicated that the promoter and coding sequence diversity of CsCCD1 might contribute to the differential accumulation of β-ionone in different tea cultivars.
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