Abstract
A clone harboring the 5′ flanking region of a tobacco glycolate oxidase (GLO) gene was isolated from a λEMBL3-tobacco genomic DNA libarary. Primer extension analysis indicated two major transcripts with 76 and 81 bp 5′ UTRs. An RT-PCR assay mapped the major mRNA transcription initiation site to thymine at position 81 upstream of the translation initiation codon. A putative TATA box spanning positions −56 to −50 upstream of the transcription initiation site was found. Promoter activity of the 5′ flanking region (−3.0 kb to +82 bp) was demonstrated in tobacco plants transformed with a GLO-β-glucuronidase (GUS) chimeric gene. Furthermore, in these transgenic plants, GUS expression patterns mimicked the expression patterns of the endogenous GLO.
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