Abstract

The ProMEX database is one of the main collection of annotated tryptic peptides in plant proteomics. The main objective of the ProMEX database is to provide experimental MS/MS-based information for cell type-specific or sub-cellular proteomes in Arabidopsis thaliana, Medicago truncatula, Chlamydomonas reinhardtii, Lotus japonicus, Lotus corniculatus, Phaseolus vulgaris, Lycopersicon esculentum, Solanum tuberosum, Nicotiana tabacum, Glycine max, Zea mays, Bradyrhizobium japonicum, and Sinorhizobium meliloti. Direct links at the protein level to the most relevant databases are present in ProMEX. Furthermore, the spectral sequence information are linked to their respective pathways and can be viewed in pathway maps.

Highlights

  • Mass spectrometry (MS)-based proteomics is the most widely used high-throughput technology for identification, quantification, and characterization of the proteins that constitute a proteome (Bensimon et al, 2012)

  • There is a large amount of sequence information for several plant species that has been generated from MS-based proteomics projects (Cao et al, 2011)

  • The spectral information of Arabidopsis thaliana is connected to the MASCP Gator (Joshi et al, 2011)

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Summary

Introduction

Mass spectrometry (MS)-based proteomics is the most widely used high-throughput technology for identification, quantification, and characterization of the proteins that constitute a proteome (Bensimon et al, 2012). There is a large amount of sequence information for several plant species that has been generated from MS-based proteomics projects (Cao et al, 2011). As a further result important meta-information has been obtained, such as sub-cellular localization and semi-quantitative protein levels, but data interpretation of complex experiments is still a challenge. The database provides access to information of previously identified modifications as well as metadata of the spectra originating experiments.

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