Prolonged Stability of Stored Vancomycin, Gentamicin, and Heparin for Use in the Antibiotic-Lock Technique

Abstract

The antibiotic-lock technique has been effective in salvaging tunneled catheters in hemodialysis patients with bacteremia. However, a practical concern exists with respect to the stability of the antibiotics and heparin in normal saline, when stored for a prolonged period. Vancomycin, gentamicin, and heparin were diluted in normal saline to a final concentration of 100 microg/ml of each antibiotic and 5000 units/ml heparin. Fresh samples, and samples refrigerated at 4 degrees C for 48 hours, 1 week, 2 weeks, 3 weeks, and 4 weeks, were assayed (in triplicate) for gentamicin and vancomycin concentration and bactericidal activity (Schlichter test) using methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. An anti-Xa activity assay was used for monitoring heparin anticoagulant activity of the fresh samples and samples refrigerated for 2 and 4 weeks. Mean (+/- SD) anti-Xa activity for heparin/vancomycin solution was 7900 +/- 173 u/ml, and for heparin/gentamicin solution was 7467 +/- 751u/ml; both were stable over a 4-week storage period. Mean bactericidal titer for vancomycin was 1:121 +/- 11, and for gentamicin was 1:242 +/- 22; both were stable over a 4-week storage period. Mean vancomycin concentration was 97 +/- 4 microg/ml, and gentamicin concentration was 86 +/- 3 microg/ml; both were stable over a 4-week storage period. Vancomycin and gentamicin in a heparin/saline solution can be stored at 4 degrees C for up to 4 weeks without adversely affecting antibiotic concentration, bactericidal activity, or heparin anticoagulant activity.