Abstract
Adult 4-day cyclic female rats of the Charles River CD strain were stimulated electrochemically in the preoptic area at either 0500 or 1100 hours on the morning of proestrus and were hypophysectomized two hours later. They were autopsied at various times from 2230 on the evening of proestrus until 0500 on the following morning. The time of ovulation for animals stimulated at either 0500 or 1100 was essentially the same. Thus the interval to ovulation was five to six hours longer in animals treated at 0500, nearly 24 hours thus being required for the ovulatory quota of gonadotropin to produce ovulation. (Endocrinology 90: 289, 1972
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