Abstract
The main goal of our research was to study the possible alterations of the chemical coding of the dorsal motor vagal nucleus (DMX) neurons projecting to the porcine stomach prepyloric region following prolonged acetylsalicylic acid supplementation. Fast Blue (FB) was injected into the studied area of the stomach. Since the seventh day following the FB injection, acetylsalicylic acid (ASA) was given orally to the experimental gilts. All animals were euthanized on the 28th day after FB injection. Medulla oblongata sections were then processed for double-labeling immunofluorescence for choline acetyltransferase (ChAT), pituitary adenylate cyclase-activating peptide (PACAP), vasoactive intestinal polypeptide (VIP), nitric oxide synthase (NOS), galanin (GAL), substance P (SP), leu enkephalin (LENK), and cocaine- and amphetamine-regulated transcript (CART). In the control DMX, only PACAP was observed in 30.08 ± 1.97 % of the FB-positive neurons, while VIP, NOS, GAL, SP, LENK, and CART were found exclusively in neuronal processes running between FB-labeled perikarya. In the ASA DMX, PACAP was revealed in 49.53 ± 5.73 % of traced vagal perikarya. Moreover, we found de novo expression of VIP in 40.32 ± 7.84 %, NOS in 25.02 ± 6.08 %, and GAL in 3.37 ± 0.85 % of the FB-labeled neurons. Our results suggest that neuronal PACAP, VIP, NOS, and GAL are mediators of neural response to aspirin-induced stomach inflammatory state.
Highlights
Acetylsalicylic acid (ASA, aspirin) is a nonsteroidal antiinflammatory drug (NSAID) which has been commonly used to relieve pain and fever
COX-2 is mainly induced by inflammatory processes, and COX-3 is a variant of COX-1 found in the nervous system (Vane and Botting 2003; Fuster and Sweeny 2011)
The Fast Blue (FB)-labeled perikarya were observed to be scattered throughout the whole extent of both left and right DMX (Gańko and Całka 2013)
Summary
Acetylsalicylic acid (ASA, aspirin) is a nonsteroidal antiinflammatory drug (NSAID) which has been commonly used to relieve pain and fever. Due to its antiplatelet action, aspirin is often applied for cardiovascular protection (Hsu 2012). ASA operates through inhibition of cyclooxygenase (COX). COX-1 is constitutively expressed in the majority of cells and in the stomach; it is directly responsible for gastric mucosa protection. COX-2 is mainly induced by inflammatory processes, and COX-3 is a variant of COX-1 found in the nervous system (Vane and Botting 2003; Fuster and Sweeny 2011). ASA is used as an antiplatelet agent and as a drug for the treatment of inflammatory processes. For the same reason, aspirin is responsible for a wide range of undesirable actions
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
