Abstract
1. The duration of motility and of fertilizing capacity of sea urchin sperm diluted in sea water can be considerably extended by the addition of any one of certain metal-chelating agents. These include ethylenediaminetetraacetate (Versene), diethyldithiocarbamate, 8-hydroxyquinoline, α-benzoinoxime and various amino acids and peptides (glycine, alanine, valine, leucine, lysine, glutamic acid, histidine, phenylalanine, tryptophane, cysteine, glycylglycine, glutathione). The relative increase in life-span, in the presence of these agents, is greater the more dilute the sperm suspension, while the absolute increase is generally greater for the more concentrated suspensions. Over 100-fold extension of life-span of dilute suspensions has been obtained by use of these agents.2. An artificial sea water of low heavy metal content also enables the sperm to survive longer than in ordinary sea water. This effect is obtained with artificial solutions in which the calcium concentration ranges from 8 times to 1/100 tha...
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