Abstract
The aim of this work was to investigate the cell proliferative activity of multi-oocyte follicles in adult sheep. Ovaries (n=64) were processed for histology and immunostaining with proliferation cell nuclear antigen (PCNA). Multi-oocyte follicles were observed in 87.50% (28/32) pairs of ovaries. Positive immunostaining for anti-PCNA antigen was observed in 97.8% of the one-oocyte follicles and 97.2% of multi-oocytes follicles (p>0.05). Oocytes included in the same follicle showed a similar pattern of PCNA staining and appear to be in the same developmental stage.
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