Proliferation of Luteal Steroidogenic Cells in Cattle

Shin Yoshioka,Hironori Abe,Ryosuke Sakumoto,Kiyoshi Okuda,Bin He

doi:10.1371/journal.pone.0084186

Shin Yoshioka, Hironori Abe + Show 3 more

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https://doi.org/10.1371/journal.pone.0084186

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Journal: PLoS ONE	Publication Date: Dec 27, 2013
Citations: 30	License type: CC BY 4.0

Affiliation: Okayama University, Institute of Agrobiological Sciences

Abstract

The rapid growth of the corpus luteum (CL) after ovulation is believed to be mainly due to an increase in the size of luteal cells (hypertrophy) rather than an increase in their number. However, the relationship between luteal growth and the proliferation of luteal steroidogenic cells (LSCs) is not fully understood. One goal of the present study was to determine whether LSCs proliferate during CL growth. A second goal was to determine whether luteinizing hormone (LH), which is known have roles in the proliferation and differentiation of follicular cells, also affects the proliferation of LSCs. Ki-67 (a cell proliferation marker) was expressed during the early, developing and mid luteal stages and some Ki-67-positive cells co-expressed HSD3B (a steroidogenic marker). DNA content in LSCs isolated from the developing CL increased much more rapidly (indicating rapid growth) than did DNA content in LSCs isolated from the mid CL. The cell cycle-progressive genes CCND2 (cyclin D2) and CCNE1 (cyclin E1) mRNA were expressed more strongly in the small luteal cells than in the large luteal cells. LH decreased the rate of increase of DNA in LSCs isolated from the mid luteal stage but not in LSCs from the developing stage. LH suppressed CCND2 expression in LSCs from the mid luteal stage but not from the developing luteal stage. Furthermore, LH receptor (LHCGR) mRNA expression was higher at the mid luteal stage than at the developing luteal stage. The overall results suggest that the growth of the bovine CL is due to not only hypertrophy of LSCs but also an increase in their number, and that the proliferative ability of luteal steroidogenic cells decreases between the developing and mid luteal stages.

Highlights

The corpus luteum (CL) is a transient endocrine gland formed from a ruptured follicle after ovulation
To elucidate whether luteal steroidogenic cells (LSCs) proliferate during CL growth, we examined 1) the expression of KI-67, a cell proliferation marker, and HSD3B, which is a marker specific for steroidogenic cells, in bovine luteal tissue, 2) the expression of cell cycle-related genes and PTEN in freshly isolated LSCs and 3) the proliferation of cultured LSCs isolated from the developing and mid CL
KI-67-positive cells were localized in the CL from the early, developing and mid luteal stages and the rate of KI-67 staining was greater in the early and developing CL than in the mid CL (Figure 1A-F)

Summary

Introduction

The corpus luteum (CL) is a transient endocrine gland formed from a ruptured follicle after ovulation. The CL produces progesterone (P4), which is required for the establishment and maintenance of pregnancy in mammals [1,2]. The CL is one of the fastest growing tissues in adult female mammals [3,4]. During the first 10 days of the 21-day bovine estrous cycle, luteal weight increases by 20- to 30-fold [4]. As the CL forms, it becomes highly vascularized and the vascularization is accompanied by a rapid proliferation of luteal endothelial cells [11,12,13]. During growth of the CL, luteal steroidogenic cells (LSCs) undergo dynamic changes [4,6], but there is no clear evidence that they proliferate

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