Abstract
AIM: To evaluate the proliferative capacity of mesenchymal cells derived from human periodontal ligament on polished and plasma-treated titanium surfaces. METHODS: Eighteen titanium disks were polished and half of them (n=9) were submitted to plasma nitriding using the cathodic cage technique. Mesenchymal cells were isolated from periodontal ligament of impacted third molars (n=2) and cultured on titanium disks (polished and nitrided) and on a plastic surface as a positive control of cell proliferation. Cell proliferation was analyzed and growth curves were constructed for the different groups by determining the number of cells adhered to the different surfaces at 24, 48 and 72 h after plating. RESULTS: Higher cell number was observed for the nitrided surface at 24 and 48 h. However, no statistically significant difference in cell proliferation was observed between the two different surface treatments (p>0.05). CONCLUSIONS: We concluded that plasma nitriding produced surfaces that permitted the proliferation of human periodontal ligament mesenchymal cells. Associated to other physical and chemical properties, it is possible to assume the feasibility of plasma nitriding method and its positive effect on the early cellular events of osseointegration.
Highlights
Received for publication: February 28, 2013 Accepted: June 25, 2013Correspondence to: Carlos Augusto Galvão Barboza Avenida Senador Salgado Filho, 3000, CEP: 59072-970 – Lagoa Nova, Natal, RN, BrasilCell cultures have been extensively used in implantology to evaluate the effect of the substrate on the behavior of osteoblasts during osseointegration
This study evaluated the proliferation of mesenchymal cells derived from the periodontal ligament of human third molars on polished and plasma-nitrided titanium surfaces
Cell adhesion to titanium surfaces is known to be the key factor for osseointegration
Summary
Received for publication: February 28, 2013 Accepted: June 25, 2013Correspondence to: Carlos Augusto Galvão Barboza Avenida Senador Salgado Filho, 3000, CEP: 59072-970 – Lagoa Nova, Natal, RN, BrasilCell cultures have been extensively used in implantology to evaluate the effect of the substrate on the behavior of osteoblasts during osseointegration. Various biological events associated with bone healing on implant surfaces can be investigated separately using appropriate cell cultures, such as cell adhesion, proliferation and differentiation, as well as the production and mineralization of extracellular matrix. The use of these cultures provides cellular and molecular data that favor a nanostructural engineering approach in implant design and permits the testing of different hypotheses. Within this context, cell cultures offer unique insights into the process and phenomenon of osseointegration[1]. The functional and structural adhesion of bone tissue to the surface of load-bearing orthopedic implants seems to be a determinant factor for in vivo success[2]
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