Proliferation enhancing effect of rice extracts on neuronal PC12 cells

Abstract

Rice is well-known as source of vitamin E, beta-glucan and gamma-oryzanol which may be useful for the treatment of Alzheimer's disease [1,2,3]. Two varieties of rice, white rice and red rice, were extracted by different methods to obtain 9 extracts as followed: S1 and S2-lyophilized rinse-water from red and white rice, S3-lyophilized water extract from white rice, S4 and S5- alcoholic extract from white and red rice, S6 and S7- cold-express extract from white and red rice, S8 and S9– supercritical fluid extract from white and red rice. All extracts were tested proliferation activity on neuronal cell (PC12) by MTT assay at dosage of 50 and 100µg/ml [4]. The results showed that all extracts had no cytotoxic activity against PC12. At dose of 50µg/ml the S1, S2, S3 and S4 extracts exhibited high proliferative effect with significant level at p<0.05 by 4 independence experiments. The S1 extract showed the highest proliferative effect at dose of 100µg/ml by 3 independence experiments. These results were shown below and they were concluded that water extracts can enhance growth of neuronal cells more than the ethanolic extracts of both types of rice.