Abstract
The aim of the study was to determine subpopulation content, proliferative activity and T-lymphocyte apoptosis level in adult mice-recipients that in the juvenile period underwent multiple transplantation of immune cells with different functional properties from opposite behavioral types of donors. Materials and methods. The study was carried out on male mice (CBAxC57BL/6) F1 (n = 190), which underwent transplantation of immune cells with definite functional properties three times starting from the age of 4–5-weeks. Phenotyping of recipients’ spleen cells was carried out by flow cytometry with monoclonal antibodies against CD3+, СD4+, CD8+. Splenocyte proliferation and apoptosis were estimated. Results. Animals that underwent threefold transplantation of immune cells from singeing opposite behavioral types of donors in the juvenile period revealed different functional properties of spleen lymphocytes in adults. The most pronounced changes were detected in the animals receiving the immune cells from donors with passive behavior type. The lymphocytes of these recipients were characterized by relatively low proliferative activity and T-mytogen sensitivity, decreased CD4+, CD8+ apoptosis level under deficient medium conditions and increased level of activation and dexamethasone-induced apoptosis in CD4+ lymphocytes.
Highlights
which underwent transplantation of immune cells with definite functional properties three times starting from the age of 4–5-weeks
most pronounced changes were detected in the animals receiving the immune cells from donors
The lymphocytes of these recipients were characterized by relatively low proliferative activity and T-mytogen sensitivity
Summary
Научно-исследовательский институт фундаментальной и клинической иммунологии (НИИФКИ) 630099, г. Цель исследования – определение субпопуляционного состава, пролиферативной активности и уровня апоптоза Т-лимфоцитов у половозрелых мышей-реципиентов, подвергнутых в ювенильный период развития многократной трансплантации иммунных клеток с различными функциональными показателями, характерными для животных с оппозитными типами поведения. Подвергнутые в ювенильный период развития многократной трансплантации иммунных клеток от сингенных доноров с оппозитными типами поведения, в половозрелом возрасте характеризуются различными функциональными свойствами лимфоцитов селезенки. Авторы декларируют отсутствие явных и потенциальных конфликтов интересов, связанных с публикацией настоящей статьи. Финансирование исследований производилось за счет средств субсидий, представляемой НИИФКИ на оказание государственных услуг, установленных государственным заданием. Пролиферация и апоптоз лимфоцитов у экспериментальных животных после многократной трансплантации клеток иммунной системы, проведенной в ювенильный период развития.
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