Abstract
Prolactin (PRL) plays an important role in regulating mammary gland development, secreting milk, and expressing milk protein genes; making it a potential genetic marker and a candidate gene for production traits in dairy animals. The aim of the study was to determine by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method the gene and genotype frequencies of PRL gene in native East Anatolian Red (EAR) cattle, which are raised as a genetic resource in Turkey. PCR-RFLP analysis involved the use of the RsaI restriction enzyme. Three patterns of fragments were obtained. The AA, AG, and GG genotype frequencies were 0.07, 0.34, and 0.59 in the cattle population, respectively. For Prolactin-RsaI (PRL-RsaI) polymorphism, the population was in Hardy-Weinberg equilibrium. Heterozygosity was found at a medium rate as 0.338 and the calculated FIS value was 0.072. Keywords : genetic resource, mammary gland, prolactin gene, PCR-RFLP
Highlights
Polymorphic genes involved in the secretion of milk are important as candidate genes, and could be used in indirect selection of livestock because of their relationships with quantitative traits (Miceikiene et al, 2006; Alipanah et al, 2008; Alfonso et al, 2012)
PRL gene polymorphisms were investigated by the PCR-RFLP method in native East Anatolian Red (EAR) cattle raised as genetic resource in Turkey
It showed the allelic and genotypic frequencies of the PRL-RsaI polymorphism region, heterozygosity and fixation index in the native EAR cattle breed; which are raised as a genetic resource in Turkey
Summary
Polymorphic genes involved in the secretion of milk are important as candidate genes, and could be used in indirect selection of livestock because of their relationships with quantitative traits (Miceikiene et al, 2006; Alipanah et al, 2008; Alfonso et al, 2012). The most important polymorphism was located and identified by RsaI endonuclease using PCR-RFLP (Mitra et al, 1995; Brym et al, 2005) These polymorphic structures have been studied by many researchers, who confirmed statistically significant associations between these polymorphic variants and milk production traits in cattle (Dybus et al, 2005; Brym et al, 2005; He et al, 2006; Alipanah et al, 2008; Mehmannavaz et al, 2009; Rorie et al, 2009; Alfonso et al, 2012; Boleckova et al, 2012; Ishaq et al, 2012; Akyuz et al, 2012; Akyuz & Cınar, 2014; Ozkan Unal et al, 2015). The results showed that PRL-RsaI(+) allele effect was significant for milk and protein yield, where the PRL-RsaI(-) allele was unfavourable for milk and protein yield, but favourable for fat yield
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