Abstract
The biodiversity of acidophilic prokaryotes was determined in three AMD ponds (pH 2.7-6.5) in Turkey (İzmir-Halıköy antimony ore enrichment plant) using 16S rRNA cloning and denaturing gradient gel electrophoresis methods. Water samples were taken two times in March 2014 and June 2015. The microbial diversity identified includes species such as Acidiphilium angustum, Acidocella sp., Ferroplasma acidiphilum, Acidithiobacillus ferriphilus, Acidithiobacillus ferrivorans, Acidiphilium rubrum, Thiomonas sp., Acidiphilium multivorum, Acidiphilium cryptum, Ferrovum myxofaciens, Acidocella aluminiidurans with the used techniques. In addition to, it has been determined that biodiversity is variable in the operating mine pools. Aciditihobacillus ferriphilus, Acidiphilium angustum, and Acidiphilium rubrum are new records for Turkey.
Highlights
Acid mine drainage (AMD) is the largest environmental problem caused by normally associated with mining activities (Garcia-Moyano et al, 2015)
The biodiversity of acidophilic prokaryotes was determined in three AMD ponds in Turkey (İzmir-Halıköy antimony ore enrichment plant) using 16S rRNA cloning and denaturing gradient gel electrophoresis methods
The microbial diversity identified includes species such as Acidiphilium angustum, Acidocella sp., Ferroplasma acidiphilum, Acidithiobacillus ferriphilus, Acidithiobacillus ferrivorans, Acidiphilium rubrum, Thiomonas sp., Acidiphilium multivorum, Acidiphilium cryptum, Ferrovum myxofaciens, Acidocella aluminiidurans with the used techniques
Summary
Acid mine drainage (AMD) is the largest environmental problem caused by normally associated with mining activities (Garcia-Moyano et al, 2015). Culture-independent methods such as 16s rRNA gene cloning, fluorescent in situ hybridization (FISH) and denaturing gradient gel electrophoresis (DGGE) are often used to investigate the diversity of microbial community that adapts to this unique environments (Gonzalez-Toril et al, 2003; Nicomrat et al, 2006; Garcia-Moyano et al, 2015). Acidophilic chemolithotrophs such as Acidithiobacillus ferrooxidans, Acidithiobacillus thiooxidans, Leptospirillium ferrooxidans have been identified in AMD which extremely low pH and high concentrations of iron, sulfates and other heavy metals (Edwards et al, 1999; Kuang et al, 2012). Our results are the first knowledge about the prokaryotic community of the selected AMD area
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