Abstract
Long-term fertilization experiments are a useful way to elucidate the impacts of fertilization on soil ecosystems. Here, we report the prokaryotic community structure in experimental field soil after 80 years of successive fertilization. Our 16S rRNA gene sequencing detected 20,996 amplicon sequence variants, including major phyla such as Proteobacteria, Acidobacteria, and Actinobacteria.
Highlights
Long-term fertilization experiments are a useful way to elucidate the impacts of fertilization on soil ecosystems
Extraction, amplicon library construction, and Illumina sequencing were conducted with three technical replicates for each soil sample
Each nonchimeric amplicon sequence variants (ASVs) was taxonomically annotated by RDP Classifier version 2.3 (6) trained with Greengenes 13_8 (7) clustered at 97% sequence identity, followed by the removal of organelle-like ASVs
Summary
Long-term fertilization experiments are a useful way to elucidate the impacts of fertilization on soil ecosystems. Extraction, amplicon library construction, and Illumina sequencing were conducted with three technical replicates for each soil sample.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
