Projection of the marginal shell of the anteroventral cochlear nucleus to olivocochlear neurons in the cat

Abstract

The marginal shell of the anteroventral cochlear nucleus is anatomically and physiologically different from its central core. Previous studies suggest that neurons in the marginal shell are well suited to encode the intensity of acoustic stimuli. To investigate the projections of the marginal shell, a focal injection (<100 nl) of a mixture of biotinylated dextran amine (BDA) and 3H-leucine was made into the marginal shell of the cat combined with injection of cholera toxin subunit-B (CTB) into the cochleas. Following a 7-day survival, the cats were perfused. Axons and swellings labeled with BDA and olivocochlear neurons labeled with CTB were immunocytochemically stained black and brown, respectively. 3H-leucine labels were visualized by autoradiography. Labeled neural structures were examined via light microscopy. We found that swellings labeled with BDA, sometimes doubly labeled with BDA and 3H-leucine, were in close apposition with dendrites and/or somata of olivocochlear neurons identified with CTB labeling. Double labeling with BDA and 3H-leucine signifies that the label was anterogradely transported. The results support the conclusion that the anteroventral cochlear nucleus projects to medial olivocochlear neurons bilaterally and to lateral olivocochlear neurons ipsilaterally. Furthermore, the results are consistent with the interpretation that the marginal shell provides a source of the above-mentioned projections. Together with information in the literature, the present anatomical results support a hypothesis that the marginal shell provides information about stimulus intensity as a part of a reflex (or feedback gain control) system comprising the cochlea, cochlear neurons, cochlear nucleus, medial olivocochlear neurons, and cochlear outer hair cells. J. Comp. Neurol. 420:127–138, 2000. © 2000 Wiley-Liss, Inc.