Proinflammatory cytokines (TNF-a, IL-1B, IL-6) and antioxidant in different stages of patients with cutaneous Leishmaniasis

Abstract

Introduction: Leishmania is a significant health problem in many parts of world. TNF-α is an essential player in infections with leishmania major, contributing to the control of the inflammatory lesion, a lesser degree and parasite killing. Inflammatory molecules (TNF-a, IL-1B and IL-6) were probably responsible for the stronger cellular recruitment and mainly seen at the site of infections. The aim of this study was evaluated of pro inflammatory cytokines and (antioxidant) in four groups of cutaneous leishmaniasis patients compared with together and normal group. These cytokines measured in 39 patients were divided into four groups of: 1) active (acute phase of treatment); 2) non-healing (received treatment for almost two years without recovery);3) healing (recovered upon treatment); and 4) healed (treatment previously received and achieved complete remission). Pro inflammatory cytokines and antioxidant about 3 of these patients from active stage to heal were specifically evaluated also. Material & Methods: IL-1β, TNFα, IL-6 in serum of patients measured by ELISA and antioxidant determined by FRAP method. Results: Antioxidant mostly decreased in groups near to normal group, but increased in non -healing group. The serum level of pro inflammatory cytokines: TNFα, increased in three groups of patients compared to normal group. Highest level of IL-1β belong to non- healing average, whether, others and normal group had zero value of IL-1β. IL-6 had highest level in non- healing, but had not significant difference in other groups. In three of these active patients which leading to health, TNFα, IL6& IL1β strongly decreased. Discussion: This Study suggestion, there is direct correlation between of pro inflammatory cytokines (IL-1B, TNF α, IL-6) and progressive of disease. Another finding shows that antioxidant level had extremely strong correlation with progresses response to drug and lead to healthy of disease