Abstract

BACKGROUND: Higher serum cytokine levels were shown to be accompanied with anemia in patients with chronic inflammatory diseases. The influence of proinflammatory cytokines on erythropoietin receptor (EpoR) messenger ribonucleic acid (mRNA) expression was investigated in this study.METHODS: Human erythroleukemia cells, TF-1 (5 x 10^6 cells/mL), were incubated with serial dilutions of interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor α (TNF-α). The expression of EpoR mRNA in TF-1 cells was measured.RESULTS: EpoR mRNA transcripts in TF-1 cells were inhibited by all three cytokines in a concentration-dependent manner. The percentage of inhibition increased with treatment duration; with the maximal inhibition appearing after 16 hours of treatment. EpoR mRNA transcripts in TF-1 cells were inhibited by 44.2 ± 1.7% with IL-6 and 49.1 ± 3.8% with TNF-α after 16-hour incubation with 200 units/mL of individual cytokine.CONCLUSION: The obtained results showed that proinflammatory cytokine-induced decrease of EpoR in erythroid progenitor cells may be one of the major causes of anemia in patients with chronic kidney and inflammatory diseases.

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