Proinflammatory cytokines in peritoneal fluid in women with peritoneal adhesions

Abstract

The objective: to investigate the level of pro-inflammatory cytokines in the peritoneal fluid in women of reproductive age with peritoneal adhesions. Materials and methods. 58 patients verified during laparoscopy were included in the study: of them, 45 patients with stages I–II of the adhesion process, and 13 – with stages III–IV of the spread of the process. The control group consisted of 30 women of reproductive age with intact reproductive function without adhesions in the pelvis, who underwent laparoscopic sterilization. The content of IL-1, I IL-2, IL-6, and tumor necrosis factor-alpha in the peritoneal fluid was analyzed. Results. Studies of the content of pro-inflammatory cytokines IL-1 (192.5±34.8 pg/ml and268.4±48.3 pg/ml), IL-2 (145.9±21.3 pg/ml and 234.8±42.5 pg/ml), IL-6 (118.7±8.4 pg/ml and698.4±22.6 pg/ml) and TNF-α (10.2±4.8 pg/ml and 29.6±2.8 pg/ml) in peritoneal fluid showed that their levels were significantly higher (p<0.01) in women with widespread stages of the adhesion process and the lowest in CG – 18.2±4.2 pg, respectively/ml, 22.4±4.3 pg/ml and 26.4±4.7 pg/ml. The level of IL-6 – 698.4±22.6 pg/ml in the peritoneal fluid of patients with III–IV stages of the adhesion process was significantly higher (p<0.01) than the level of IL-6 – 118.7±8.4 pg/ml in the peritoneal fluid of women with stage I–II spread of adhesions and in the control group – 26.4±4.7 pg/ml. Сonclusions. With the increase in the prevalence of the adhesion process, there is a significant reliable increase in the content of cytokines in the peritoneal fluid, while the peritoneal fluid plays a significant role in its formation.