Progressive resistance training and High Intensity Interval Training increases the expression of TRAF6 gene and reduces serum levels of TNF-α in male Wistar diabetic rats

Abstract

Background: The most common causes of mortality in diabetic patients are cardiovascular disorders, one of the reasons being inflammatory factors. Given that physical activity can reduce inflammation, the present study was to investigate the effects of two types of high intensity Interval and progressive resistance training on the expression of TRAF6 and serum levels of TNF-α in male diabetic rats. Methods: In this experimental study, 72 male rats were divided into 6 groups of 12 (diabetic rats, control, diabetic high intensity Interval training, high intensity Interval training, diabetic progressive resistance training and progressive resistance training). Progressive resistance training was performed in a 6-weekly 3-session, climbing the vertical ladder, with 50%, 75%, 90% and 100% the body weight of the animal. After successful completion, 30 grams were added to the weights, to the extent that the samples can not carry the ladder. High intensity Interval training were also performed at 6-weekly 3-session, with an intensity of 50 to 110% of the maximum oxygen consumption. TRAF6 gene expression was measured by Real Time PCR. Results: TRAF6 levels increased significantly in both types of training, in diabetic group, which was higher in the interval group. TNF-α decreased significantly after both types of training, which was more prominent in the interval group. Conclusion: Both types of training reduced the TNF-α inflammatory factor and increased the expression of TRAF6 gene expression. These effects were more frequent in the interval group.