Abstract
AbstractSamples of the Douglas-fir bark beetle, Dendroctonus pseudotsugae Hopk., taken from two different segments of the infested bole were different in gallery production, egg deposition, and yolk deposition. The same two groups were distinguished by comparing fat body cell nuclei taken before the onset of the reproductive period. Techniques for comparing nuclei involved creation and analysis of digital images of nuclei. These groups were indistinguishable from each other, at that stage, by any other known method. The two groups differed in total absorbance of Fuelgen-stained nuclei (DNA content) and nuclear area. Significance of these findings is discussed in relation to early detection of reproduction-related quality differences and population dynamics of bark beetles.
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