Abstract
The plant cell wall (CW) compartment, or apoplast, is host to a highly dynamic proteome, comprising large numbers of both enzymatic and structural proteins. This reflects its importance as the interface between adjacent cells and the external environment, the presence of numerous extracellular metabolic and signaling pathways, and the complex nature of wall structural assembly and remodeling during cell growth and differentiation. Tomato fruit ontogeny, with its distinct phases of rapid growth and ripening, provides a valuable experimental model system for CW proteomic studies, in that it involves substantial wall assembly, remodeling, and coordinated disassembly. Moreover, diverse populations of secreted proteins must be deployed to resist microbial infection and protect against abiotic stresses. Tomato fruits also provide substantial amounts of biological material, which is a significant advantage for many types of biochemical analyses, and facilitates the detection of lower abundance proteins. In this review, we describe a variety of orthogonal techniques that have been applied to identify CW localized proteins from tomato fruit, including approaches that: target the proteome of the CW and the overlying cuticle; functional “secretome” screens; lectin affinity chromatography; and computational analyses to predict proteins that enter the secretory pathway. Each has its merits and limitations, but collectively they are providing important insights into CW proteome composition and dynamics, as well as some potentially controversial issues, such as the prevalence of non-canonical protein secretion.
Highlights
Tomato (Solanum lycopersicum), one of the world’s most important horticultural crops, is recognized as the pre-eminent experimental model to study fleshy fruit development, physiology, and pathology (Klee and Giovannoni, 2011; Tomato Genome Consortium, 2012; Seymour et al, 2013)
Of the various plant subcellular proteomes that have been studied, arguably the most technically challenging is that of the cell wall (CW), for the reasons described above
There are still no reports of large scale proteomic profiling initiatives of the tomato fruit CW spanning the various stage of fruit development and ripening in a single study and this likely reflects the major technical hurdles
Summary
Tomato (Solanum lycopersicum), one of the world’s most important horticultural crops, is recognized as the pre-eminent experimental model to study fleshy fruit development, physiology, and pathology (Klee and Giovannoni, 2011; Tomato Genome Consortium, 2012; Seymour et al, 2013). Of the various plant subcellular proteomes that have been studied, arguably the most technically challenging is that of the CW, for the reasons described above.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
