Abstract
Cloning of interest DNA is necessary for functional analysis of genome sequences and research on metabolically engineered pathways of modern producer strains. Methods to precisely clone large DNA fragments include library constructing and screening,PCR amplification and various different DNA assembly methods in vitro. In addition,direct cloning by homologous recombination in vivo has more advantages in the cloning and engineering of long DNA sequences. The main methods of direct cloning of large DNA fragments in vivo via Red / ET recombination system and its applications were introduced.
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