Progress in the study on genes encoding enzymes involved in bio-synthesis of very long chain fatty acids and cuticular wax in plants

Abstract

Very long chain fatty acids (VLCFAs) play a comprehensive role in organisms. They are essential biological components in seed storage triacylglycerols (TAGs), membrane lipids, and sphingolipids. They also serve as precursors of wax layer compounds. The cuticle covers the aerial surface of land plants, which consists of cutin and wax. The wax, including amorphous intracuticular wax embedded in cutin polymer and epicuticular wax crystalloids that cover the outer plant surface, plays crucial roles in plant growth and development, and adaptation to environment. Biosynthesis of VLCFAs is catalyzed by the fatty acyl-CoA elongase, a membrane-bound enzymatic complex containing 3-ketoacyl-CoA synthase (KCS), 3-ketoacyl-CoA reductase (KCR), 3-hydroxacyl-CoA dehydratase (HCD), and trans-2, 3-enoyl-CoA reductase (ECR). Very long chain fatty acid wax precursors flux into cuticular wax biosynthetic pathways through acyl reduction and decarbonylation, and then are converted to all kinds of wax components. This article reviews the functions of VLCFAs and cuticular wax, and the recent progress in cloning and characterization of genes encoding enzymes involved in catalyzing VLCFAs and cuticular wax biosynthesis. The problems existing in researches of wax genes are also discussed.