Progress in Application of Metagenomic Next-Generation Sequencing in Diagnosis and Treatment of Periprosthetic Knee Joint Infection

Abstract

Background: With the development of total knee arthroplasty, patients benefit from the occurrence of postoperative complications that affect the efficacy of the procedure. Among them, periprosthetic knee joint infection is a catastrophic complication of total knee arthroplasty, which is more difficult to diagnose and treat in the clinic and has low patient satisfaction. The ability to clearly diagnose and identify the pathogen at an early stage is an important guide to treatment and is the key to successful treatment. Objective: Traditional detection techniques have the disadvantages of low detection rate, complicated operation and time consuming in the detection of pathogens in periprosthetic knee joint infection, which are difficult to meet the needs of disease detection. Metagenomic next-generation sequencing technology is used to extract the genetic material of pathogens in samples and combine with high-throughput sequencing technology and bioinformatics analysis for detection, which has the advantages of efficient, accurate and sensitive detection of pathogens. Conclusion: Metagenomic next-generation sequencing technology has high application value in the diagnosis and treatment of infectious diseases, and its application in the field of periprosthetic knee joint infection is becoming more and more widespread, and the application of this technology in the diagnosis and treatment of periprosthetic knee joint infection is reviewed.