Abstract
This is a review article on protein detection methods based on CRISPR-Cas system. This article introduces the application of CRISPR-Cas system in the field of gene editing, and then focuses on the protein detection technology based on CRISPR-Cas system. These technologies take advantage of the targeting and high efficiency of the CRISPR-Cas system, combined with detection methods such as fluorescence or mass spectrometry, to detect a variety of proteins with high sensitivity. This article also compares the advantages and disadvantages of these techniques and traditional protein detection methods, and discusses the application prospects of these techniques in clinical diagnosis and biological research.
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