Prognostic value of serum procollagen III peptide and type IV collagen in patients with biliary atresia

Abstract

Background/Purpose: Progressive hepatic fibrosis, in spite of a successful Kasai procedure, is a major problem in patients with biliary atresia (BA). Early identification of patients at risk would be of great value. N-terminal procollagen-III peptide (PIIIP) (which is a marker of fibrogenesis and, therefore, of on going inflammation), and type IV collagen (found in basement membrane extracellular matrix), were measured in patients with BA to determine their potential as prognostic markers. Methods: Thirty-three postoperative BA patients (11.0 ± 3.7 years old) and 20 normal controls (10.5 ± 2.8 years old) were studied. The BA patients were classified on the basis of their current liver function test results into three outcome groups. Group I (n = 9) had severe liver dysfunction, group II (n = 13) had moderate, and group III (n = 11) had good liver function. Serum P-III-P and type IV collagen values were determined by radioimmunoassays and one step sandwich enzyme immunoassay. Results: In group I, serum PIIIP (1.93 ± 0.64 U/mL) and type IV collagen levels (363.5 ± 69.5 ng/mL) were significantly higher than in group II (PIIIP [1.32 ± 0.25 U/mL], type IV collagen [225.3 ± 45.4 ng/mL]; P < .01). There were increased levels in serum PIIIP and serum type IV collagen in group II compared with group III (PIIIP [1.01 ± 0.25 U/mL], type IV collagen [171.3 ± 47.2 ng/mL]; P < .01). There were no significant differences in serum PIIIP and type IV collagen levels between group III and controls. Conclusion: The authors conclude that serum levels of PIIIP and type IV collagen may be useful in the long-term follow-up of BA patients after Kasai's portoenterostomy.