Abstract
BackgroundFamily with sequence similarity 3, member C (FAM3C) has been identified as a novel regulator in epithelial-mesenchymal transition (EMT) and metastatic progression. However, the role of FAM3C in esophageal squamous cell carcinoma (ESCC) remains unexplored. The purpose of present study is to illustrate the role of FAM3C in predicting outcomes of patients with ESCC.MethodsFAM3C expression was measured in ESCC tissues and the matched adjacent nontumorous tissues by quantitative real-time RT-PCR and Western blot analysis. The relationship between FAM3C expression and prognosis of ESCC patients was further evaluated by univariate and multivariate regression analyses. Univariate and multivariate analyses of the prognostic factors were performed using Cox proportional hazards model.ResultsThe FAM3C mRNA expression was remarkably upregulated in ESCC compared with their nontumor counterparts (P < 0.001). In addition, high expression of FAM3C was significantly associated with pT stage (P = 0.014) , pN stage (P = 0.026) and TNM stage (P = 0.003). Kaplan-Meier analysis showed that the 7-year overall survival rate in the group with high expression of FAM3C was poorer than that in low expression group (32.0 versus 70.9 %; P < 0.001). Univariate and multivariate analyses demonstrated that FAM3C was an independent risk factor for overall survival. Moreover, Stratified analysis revealed that FAM3C expression could differentiate the prognosis of patients in early clinical stage (TNM stage I-II).ConclusionsFAM3C expression was dramatically increased in ESCC and might serve as a valuable prognostic indicator for ESCC patients after surgery.
Highlights
With sequence similarity 3, member C (FAM3C) has been identified as a novel regulator in epithelial-mesenchymal transition (EMT) and metastatic progression
Expression of FAM3C in esophageal squamous cell carcinoma (ESCC) Our previous RNA-seq data showed that FAM3C was overexpressed in all three tested ESCC tumor tissues compared to corresponding nontumor tissues
Western blot analysis of FAM3C expression was performed in the paired ESCC and the nontumor specimens of 12 randomly selected ESCCs from the 40 cases described above
Summary
With sequence similarity 3, member C (FAM3C) has been identified as a novel regulator in epithelial-mesenchymal transition (EMT) and metastatic progression. The role of FAM3C in esophageal squamous cell carcinoma (ESCC) remains unexplored. With sequence similarity 3, member C (FAM3C), or interleukinlike epithelial-mesenchymal transition inducer (ILEI) brought up our attention due to its significantly upregulated expression in ESCC specimens. It belongs to the family with sequence similarity 3 (FAM3) superfamily and encodes a secreted protein with a GG domain. Recent works have revealed that FAM3C was a novel regulator of epithelial-mesenchymal transition (EMT) and metastatic progression [10, 11]. The expression pattern and clinical significance of FAM3C in ESCC has not been explored
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