Abstract
We examined the prognostic significance of Epidermal Growth Factor-like repeats and Discoidin I-Like Domains 3 (EDIL3) expression and its correlations with mesenchymal phenotype and microvessel density in non-small cell lung carcinoma (NSCLC). A total of 268 NSCLC specimens were evaluated retrospectively by immunohistochemical staining for EDIL3, EMT markers (e-cadherin, β-catenin, and vimentin), and CD31 to measure microvessel density. EDIL3, e-cadherin, β-catenin, and vimentin were expressed in 16%, 22.8%, 3.7%, and 10.1% of the specimens, respectively. The mRNA level of EDIL3 in tumor was correlated with the level of EDIL3 protein expression using immunohistochemistry. In lung adenocarcinoma patients, EDIL3 expression was significantly correlated with low e-cadherin expression, high vimentin expression, and increased microvessel density (P < 0.001, P = 0.001, and P = 0.023, respectively). In lung squamous cell carcinoma patients, EDIL3 expression was significantly correlated with low e-cadherin expression and high vimentin expression (P = 0.021 and P = 0.002, respectively). In lung adenocarcinoma patients, EDIL3 was an independent prognostic factor for overall survival in a multivariate analysis (hazard ratio: 2.552, P = 0.004). EDIL3 is significantly correlated with mesenchymal phenotype, angiogenesis, and tumor progression in lung adenocarcinoma.
Highlights
The epithelial–mesenchymal transition (EMT) is a mechanism by which differentiated epithelial cells adopt a mesenchymal phenotype with loss of cell-cell junctions and loss of cellular polarity[12]
Associations among EDIL3, mesenchymal phenotype, and angiogenesis have been observed in several malignancies[11, 15], no study has examined the relationships among EDIL3, mesenchymal phenotype, and angiogenesis or the prognostic significance of EDIL3 expression in non-small cell lung carcinoma (NSCLC) patients
Our study provides several novel findings. It shows the correlation between EDIL3 expression and mesenchymal phenotype, characterized by low e-cadherin and enhanced vimentin expression in NSCLC patients
Summary
Dongjun Jeong[1], Seona Ban[2], Seunghyun Oh2, Su Jin Lee[3], Seong Yong Park4 & Young Wha Koh[5]. We examined the prognostic significance of Epidermal Growth Factor-like repeats and Discoidin I-Like Domains 3 (EDIL3) expression and its correlations with mesenchymal phenotype and microvessel density in non-small cell lung carcinoma (NSCLC). EDIL3 expression was significantly correlated with low e-cadherin expression, high vimentin expression, and increased microvessel density (P < 0.001, P = 0.001, and P = 0.023, respectively). Overexpression of the EDIL3 gene enhances the features of EMT15 It promotes cancer cell proliferation and invasion in a non-small lung carcinoma cell line[16]. The objective of this retrospective study was to evaluate the expression levels of EDIL3, the EMT markers e-cadherin, β-catenin, and vimentin, and microvessel density (MVD) using CD31 in NSCLC patients via immunohistochemical staining to determine the correlations among these markers and assess the prognostic significance of EDIL3 expression in NSCLC
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