Abstract
Currently, there is a lack of blood markers for the detection of recurrent oral squamous cell carcinoma (OSCC). The present study aimed to investigate whether the aberrant expression of single microRNAs (miRNAs) in whole blood of patients could serve as a biomarker for persistent or recurrent OSCC. Whole blood of 2groups of formerly treated OSCC patients was investigated by RT-qPCR for their circulating miRNA profiles. The R-OC group included patients with recurrence of OSCC (n=21) and the NR-OC group included patients without recurrence (n=21). Fold-changes and significance of the differences in miRNA expression levels between the groups were determined. A cut-off point (COP) for the discrimination between the R-OC and NR-OC groups was calculated and the significance between over/under expression of the miRNAs and the recurrence of malignancy was determined. Significant differences in the miRNA expression in whole blood of the R-OC and NR-OC groups were found. The levels of miR-3651 and miR-494 were significantly increased and the level of miR-186 was significantly decreased in whole blood of the R-OC patients (pmiR-3651=0.001, pmiR-494=0.003 and pmiR-186=0.001). By the determination of the COP, increased or decreased expression of the markers was significantly correlated to the recurrence of the disease. Altered expression of miR-494, miR-3651 and miR-186 appears to be associated with the recurrence of OSCC. The present study may form the basis for establishing a blood test as a minimally invasive method for the detection of the recurrence of OSCC.
Highlights
Late diagnosis, frequent regional lymph node metastases and the high rate of local and regional recurrence that is causedKey words: OSCC, recurrence, whole peripheral blood, miRNA expression, Real-time quantitative reverse transcription-PCR (RT-qPCR) mainly by the persistence of malignant cells, are the major causes for the poor prognosis of oral squamous cell carcinoma (OSCC) [1,2]
Even following wide tumor excision, a local recurrence of OSCC occurs in up to 30% of OSCC patients due to residual malignant cells that could not be microscopically detected in tumor margins at the time of surgery [9]
The present study aimed to evaluate the difference in circulating miRNA expression in whole blood samples of OSCC patients suffering from recurrence compared to patients who were disease-free for at least 1 year after treatment in order to clarify their impact in the early detection of recurrent disease
Summary
Frequent regional lymph node metastases and the high rate of local and regional recurrence that is caused. Overexpression of miR-3651 was associated to lymph node metastases, clinical stage and to more dedifferentiated tumors These miRNAs may be useful in diagnostic and prognostic applications [31,32]. The present study aimed to evaluate the difference in circulating miRNA expression in whole blood samples of OSCC patients suffering from recurrence compared to patients who were disease-free for at least 1 year after treatment in order to clarify their impact in the early detection of recurrent disease
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