Abstract
Endometrial cancer is one of the most common malignancies of the reproductive female tract, with endometrioid endometrial cancer being the most frequent type. Despite the relatively favourable prognosis in cases of endometrial cancer, there is a necessity to evaluate clinical and prognostic utility of new molecular markers. MiRNAs are small, non-coding RNA molecules that take part in RNA silencing and post-transcriptional regulation of gene expression. Altered expression of miRNAs may be associated with cancer initiation, progression and metastatic capabilities. MiRNA-205 seems to be one of the key regulators of gene expression in endometrial cancer. In this study, we investigated clinical and prognostic role of miRNA-205 in endometrioid endometrial cancer. After total RNA extraction from 100 archival formalin-fixed paraffin-embedded tissues, real-time quantitative RT-PCR was used to define miRNA-205 expression levels. The aim of the study was to evaluate miRNA-205 expression levels in regard to patients’ clinical and histopathological features, such as: survival rate, recurrence rate, staging, myometrial invasion, grading and lymph nodes involvement. Higher levels of miRNA-205 expression were observed in tumours with less than half of myometrial invasion and non-advanced cancers. Kaplan-Maier analysis revealed that higher levels of miRNA-205 were associated with better overall survival (p = 0,034). These results indicate potential clinical utility of miRNA-205 as a prognostic marker.
Highlights
Endometrial cancer (EC) is one of the most common malignancies among women worldwide
Expression of miRNA-205 was greatly upregulated in the endometrial cancer (EEC) specimens in comparison to normal endometrium samples (p = 0,000158, Fig 2)
RQ values, which were evaluated in the EEC tissues derived from 90 patients, were used to determine miRNA-205 clinical significance
Summary
Endometrial cancer (EC) is one of the most common malignancies among women worldwide. Due to the longer expected life duration, lifestyle diseases and increase of obesity rates, EC has become a major oncological issue in developed countries [1].
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