Abstract
Uterine cytosol prepared from estrogen treated rabbits was stored for various lengths of time at −60°C. The avidity for [ 3H]-progesterone, the number of progesterone binding sites, and the relative binding affinities of five steroids were determined for fresh cytosol, and for cytosol subjected to freezer storage. None of the binding parameters differed from those determined for fresh cytosol after 1, 2, 4, 5 or 8 weeks of storage. However, after 16 weeks of storage the relative binding affinities of three steroids had increased significantly. Rabbit uterine cytosol may be stored at −60°C for 2 months without any perceptible change in the binding properties of the progesterone receptor.
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