Profiling of Volatile Organic Compounds in Wild Indigenous Medicinal Ginger (Zingiber barbatum Wall.) from Myanmar.

Musavvara Kh Shukurova,Yonathan Asikin,Miyako Kusano,Kazuo N Watanabe,Yanhang Chen

doi:10.3390/metabo10060248

Musavvara Kh Shukurova, Yonathan Asikin + Show 3 more

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https://doi.org/10.3390/metabo10060248

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Abstract

The emissions of volatile organic compounds (VOCs) strongly depend on the plant species and are differently represented in specific taxa. VOCs have a degree of chemical diversity and also can serve as chemotaxonomic markers. Zingiber barbatum Wall. is a wild medicinal ginger plant endemic to Myanmar whose VOC composition has never been screened before. In this study, we screened the rhizome of Z. barbatum to identify the VOC composition by the application of gas chromatography combined with time-of-flight-mass spectrometry (GC-TOF-MS). The resulting VOC profile of Z. barbatum showed that it consists mainly of monoterpenes (21%) and sesquiterpenes (30%). Intraspecific similarities and dissimilarities were found to exist between Z. barbatum genotypes in terms of VOC composition. Four accessions (ZO191, ZO223, ZO217, and the control accession ZO105) collected from the Shan State and Mandalay region of Myanmar were found to share a similar VOC profile, while two accessions (ZO64 and ZO160) collected from the Bago region were found to vary in their VOC profiles compared with the control accession. The two identified compounds, i.e., α-bergamotene and β-(E)-guaiene may serve as discriminative chemical markers for the characterization of Z. barbatum species collected in these three geographical regions of Myanmar. This study represents a first attempt to identify and describe the VOCs in the medicinal species Z. barbatum that have not been reported to date.

Highlights

Volatile organic compounds (VOCs) are involved in various vital activities of plants
The Headspace solid-phase microextraction (HS-solid-phase microextraction (SPME)) approach was applied for non-targeted gas chromatography (GC)-TOF-mass spectra (MS) profiling of the VOC composition in Z. barbatum species collected from three different eco-geographical regions of Myanmar
This approach was shown to be suitable for profiling the VOC composition in Z. barbatum species A total of 21% of the identified VOCs were found to be monoterpenoids, and a total of 30% of the identified VOCs were found to be sesquiterpenoids

Summary

Introduction

Volatile organic compounds (VOCs) are involved in various vital activities of plants. VOCs have a high degree of chemical diversity and are differently represented in specific taxa; they can serve as chemotaxonomic markers [3,6]. Based on their biosynthetic origin and chemical structure, the major classes of plant VOCs are volatile terpenes (such as monoterpenes, sesquiterpenes, diterpenes, and isoprenes) and oxygenates (such as aldehydes, alcohols, ketones, and esters), phenylpropanoids, derivatives of fatty acids and amino acids, and moderately volatiles compounds, such as furanocoumarins and their derivatives [3,7,8]

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